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Author: search.filters.author.Roy, S.C.Has files: Yes

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    Characterization of Rice [Oryza saliva L.] germplasm based on iron and zinc content
    (University of North Bengal, 2013-03) Roy, S.C.; Sharma, B.D.; Singha, S.; Sinha, B.
    Hidden hunger is caused by the consumption of food grains (rice) which are deficient in mineral micronutrients specifically iron and zinc. This hunger affects more than one-half of the world's population especially woman and children in developing countries causing anemia and child blindness because 60 per cent of the world's population is dependent on rice as their staple food. Considering this biofortification as one of the suitable approaches was considered for improving the Fe and Zn content and their bioavailability in rice grain. Local landraces of rice were collected and Fe and Zn content were quantified for their genotypic characterization. Iron (Fe) and Zinc (Zn) content of 112 local landraces were estimated according to Lindsey and Norwell by using Atomic Absorption Spectrophotometer Varian Speetra AA 50B. Iron concentration ranged from 0.25μg/g to 3.25μg/g and zinc from 0.85μg/g to 195.3μg/g in the landraces. Highest iron containing rice was Swetonunia with 34.8μg/g and highest Zn was found in Nepali Kalam which was 195.3μg/g. Zn content is quite comparable to other HYVs and can be used as donor parent in breeding program for biofortification of micronutrient Zn.
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    ItemOpen Access
    Bacterial strain isolated from the Mango field and identified on the basis of 16S rDNA sequence information and optimization of the alkaline cellulase production
    (University of North Bengal, 2008-03) Roy, S.C.; Chattopadhyay, A.
    A bacterial strain was isolated from the soil of mango (Mangifera indica) orchards. The isolate was identified as Paenibacillus ahvei [MTCC 7809] through phylogenetic analysis based on the 16S rDNA gene sequencing and also by conventional biochemical processes. The scanning electron microscopic analysis revealed the morphological details of the strain. The isolate is Gram positive. motile, rod shaped, spore forming bacterium with extreme salt tolerance. It can hydrolyze starch and gelatin, and catalase and oxidase were produced. The cellulase activity of the culture broth was determined by measuring the reducing sugar released from carboxymethyl cellulose (CMC). Different cultural conditions were tested to reach the optimum growth conditions in order to obtain large-scale production of alkaline cellulase for commercial importance. Crude cellulase from Paenibacillus alvei (MTCC 7809) showed activity and stability at moderately high temperature and pH and the activity remained stable at 40°C and pH 8 after 2h of incubation making the strain a good choice for industrial applications. The rDNA sequence of 1509bp is submitted into the NCBI GenBank database (Acession no. EF429201).