Bacterial strain isolated from the Mango field and identified on the basis of 16S rDNA sequence information and optimization of the alkaline cellulase production
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Type
Article
Date
2008-03
Journal Title
NBU Journal of Plant Sciences
Journal Editor
Saha, A
Journal ISSN
Volume Title
Publisher
University of North Bengal
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Roy, S. C., & Chattopadhyay, A. (2008). Bacterial strain isolated from the Mango field and identified on the basis of 16S rDNA sequence information and optimization of the alkaline cellulase production. NBU Journal of Plant Sciences, 2, 67–76. https://ir.nbu.ac.in/handle/123456789/4401
Authors
Roy, S.C.
Chattopadhyay, A.
Advisor
Editor
Abstract
A bacterial strain was isolated from the soil of mango (Mangifera indica) orchards. The isolate
was identified as Paenibacillus ahvei [MTCC 7809] through phylogenetic analysis based on the 16S
rDNA gene sequencing and also by conventional biochemical processes. The scanning electron
microscopic analysis revealed the morphological details of the strain. The isolate is Gram positive.
motile, rod shaped, spore forming bacterium with extreme salt tolerance. It can hydrolyze starch and
gelatin, and catalase and oxidase were produced. The cellulase activity of the culture broth was determined
by measuring the reducing sugar released from carboxymethyl cellulose (CMC). Different cultural
conditions were tested to reach the optimum growth conditions in order to obtain large-scale production
of alkaline cellulase for commercial importance. Crude cellulase from Paenibacillus alvei (MTCC 7809)
showed activity and stability at moderately high temperature and pH and the activity remained stable at
40°C and pH 8 after 2h of incubation making the strain a good choice for industrial applications. The
rDNA sequence of 1509bp is submitted into the NCBI GenBank database (Acession no. EF429201).
Description
Citation
Accession No
Call No
Book Title
Edition
Volume
ISBN No
Volume Number
2
Issue Number
ISSN No
0974-6927
eISSN No
Pages
Pages
67 - 76