Serological and molecular detection of Macrophomina phaseolina, causing root rot of Citrus reticulata
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Type
Article
Date
2012-03
Journal Title
NBU Journal of Plant Sciences
Journal Editor
Sen, Arnab
Journal ISSN
Volume Title
Publisher
University of North Bengal
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Chakraborty, B. N., Chakraborty, U., Rai, K., Sunar, K., & Dey, P. L. (2012). Serological and molecular detection of Macrophomina phaseolina, causing root rot of Citrus reticulata. NBU Journal of Plant Sciences, 6(1), 77–86. https://ir.nbu.ac.in/handle/123456789/4459
Authors
Chakraborty, B.N.
Chakraborty, U.
Rai, K.
Sunar, K.
Dey, P.L.
Advisor
Editor
Abstract
Polyclonal antibodies (PAbs) were raised against mycelial antigens of Macrophomina phaseolina a causal organism of root rot disease of mandarin plants. IgG was purified and further packaged into immunological formats such as immuno diffusion, Plate trapped antigen (PTA)-ELISA, dot immunobinding assay, Western blot analysis and indirect immunofluorescence for quick and accurate detection of pathogen from soil. Indirect staining of mycelia and sclerotia of M. phaseolina with homologous PAb and labeling with goat antirabbit IgG conjugated with FITC developed strong fluorescence in young hyphal tips and sclerotia of M. phaseolina. Genomic DNA prepared from mycelia of M. phaseolina was purified and PCR amplification of 18S rDNA was done using ITS region specific primer pair. The amplified DNA was sequenced and aligned against ex-type strain sequences from NCBI GenBank using BLAST and phylogenetic analysis was obtained using MEGA4 software. Amplification of ITSI region of the rDNA can be considered as a rapid technique for identifying pathogens successfully in all cases.
Description
Citation
Accession No
Call No
Book Title
Edition
Volume
ISBN No
Volume Number
6
Issue Number
1
ISSN No
0974-6927
eISSN No
Pages
Pages
77 - 86