Please use this identifier to cite or link to this item: http://ir.nbu.ac.in/handle/123456789/4417
Title: Pathogenesis-related proteins of tea triggered by Exobasidium vexans
Other Titles: Journal of Plant Sciences,Vol.3, (March 2009) p 59-66
Authors: Chakraborty, BN
Sharma, M
Das Biswas, R
Ghosh, AN
Keywords: Blister blight
PR- 2
PR -3
Defense enzymes
Immunogold labeling
Issue Date: Mar-2009
Publisher: University of North Bengal
Abstract: The defense strategy of tea plants against Exobasidium vexans are multifold and include accumulation of pathogenesis-related (PR) proteins. A study on the association of defense enzymes with resistance in tea plants triggered by E. vexans revealed significant changes in the level of β-1.3-glucanase (PR 2) and chitinase (PR 3) exhibiting antimicrobial activity. Accumulation of defense proteins differed in time and magnitude. Time course studies points towards accumulation of PR-2 and PR-3 in the early hours, PR-9 later on and finally the antifungal metabolites that confer resistance to the plants. Treatment with salicylic acid (SA) stimulates a multicomponent defense response in tea leaves which was confirmed by immuno-localization of PR 2 and PR 3 in tea leaf tissues following induction of resistance. Induction of PR-3 in suspension-cultured tea cells following SA treatment was confirmed immunologically using antibody probes (PAb-chitnase). Subcellular localization of PR-3 and PR-2 in tea leaves were also confirmed by indirect immunogold labeling. Marked increase in frequency of gold particles following elicitation by SA treatment was evident. Cell defense responses associated with systemic acquired resistance induced by SA against E.vexans has been discussed in relation to the possible role of PR-proteins in immunizing tea plants
URI: http://ir.nbu.ac.in/handle/123456789/4417
ISSN: 09746927
Appears in Collections:NBU Journal of Plant Sciences, Vol.3, (March 2009)

Files in This Item:
File Description SizeFormat 
NBU Journal of Plant Sciences, Vol. 3, March 2009_11.pdfPathogenesis-related proteins of tea triggered by Exobasidium vexans758.86 kBAdobe PDFThumbnail
View/Open


Items in NBU-IR are protected by copyright, with all rights reserved, unless otherwise indicated.