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Subject: search.filters.subject.HPLC

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    Comparative analysis of antioxidant activities and phytochemical properties of some culinary herbs
    (University of North Bengal, 2016-03) Dev Chaudhuri, Sandip; Shah, Anju; Chakraborty, Usha
    The present work aimed to evaluate the antioxidant activities as well as phytochemical analysis of leaf extracts of some commonly used leafy spices such as Murraya koenigi (Mk), Coriandrum sativum (Cs), Trigonella foenum-graecum (Tfg) and Mentha x piperita (Mp). Lyophilised plant extracts (LPEs) were obtained by hot water extraction (HWE) process followed by rotavap and lyophilisation. Among the herbs tested, Mk showed the highest antioxidant activity in DPPH Scavenging (77.35 % mg-1 of LPE), superoxide anion radical scavenging (60.21 % mg-1 of LPE) and hydrogen peroxide scavenging (57.21 % mg-1 of LPE) model. Tfg showed least activity in DPPH scavenging (33.15 % mg-1 of LPE) and superoxide anion radical scavenging (25.36 % mg-1 of LPE) assay while Cs had the least activity in hydrogen peroxide scavenging (43.70 % mg-1 of LPE) system. Phytochemical investigations revealed the presence of major primary and secondary metabolites. MK possessed highest amount of phenolics (5.70 mg GAE g-1 of LPE), soluble sugars (68.18 mg GLE g-1 of FTW) and proteins (69.84 mg BSAE g-1 of FTW) and plant pigments (total chlorophyll 6.22 mg g-1 of FTW and total carotenoid 0.19 μg g-1 of FTW) among the herbs. SDS-PAGE and HPLC finger printing had been performed for analysis of protein patterns and phenolic compounds respectively. In conclusion, addition of culinary herbs and leafy spices that show high to moderate antioxidant activity with an excellent amount of phytochemicals in dietary items would go a long way in assuring human health and wellness as well as enhancement of the disease fighting capacity against oxidative stress related disorders.
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    ItemOpen Access
    A comparative study of silkworm (Bombyx mori L.) rearing under different sources of peptides isolated from Dudhiya and S1 mulberry leaves
    (University of North Bengal, 2015-03) Jha, Suchisree; Bhattacharyya, Phalguni; Ghosh, Amitava; Mandal, Palash
    Silkworm is a domestic monophagous insect, produces only natural animal the fibre. Growth of silkworm larvae depends on nutritional components of mulberry leaves. In present study one attempt was made to investigate the role of low molecular weight (0.5-3 kDa) mulberry peptides on silkworm rearing. For peptide extraction, two different types of mulberry leaves were chosen, one from S1 which was preferred by larvae for feeding, another is a germplasm named Dudhia, refused by larvae. Peptides isolated from young S1 leaves showed higher larval growth followed by peptides isolated from mature and senescence leaves. High ERR% along with enhanced weight of single cocoon and single shell was observed in silkworm fed with S1 peptide treated mulberry leaves as compared with the same by Dudhia peptides. Elevated antioxidant activities were exhibited by S1 peptides than Dudhia at all maturity stages (young, mature and senescence). Significant correlation was obtained between antioxidant activities of S1 peptides and economical attributes of silkworm rearing such as ERR %, weight of single cocoon, weight of single shell etc. From our observation it might be stated that the farmers would have been benefited if they could use mulberry leaves treated with antioxidant enriched peptides as a food for silkworm rearing.