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    Characterization of Rice [Oryza saliva L.] germplasm based on iron and zinc content
    (University of North Bengal, 2013-03) Roy, S.C.; Sharma, B.D.; Singha, S.; Sinha, B.
    Hidden hunger is caused by the consumption of food grains (rice) which are deficient in mineral micronutrients specifically iron and zinc. This hunger affects more than one-half of the world's population especially woman and children in developing countries causing anemia and child blindness because 60 per cent of the world's population is dependent on rice as their staple food. Considering this biofortification as one of the suitable approaches was considered for improving the Fe and Zn content and their bioavailability in rice grain. Local landraces of rice were collected and Fe and Zn content were quantified for their genotypic characterization. Iron (Fe) and Zinc (Zn) content of 112 local landraces were estimated according to Lindsey and Norwell by using Atomic Absorption Spectrophotometer Varian Speetra AA 50B. Iron concentration ranged from 0.25μg/g to 3.25μg/g and zinc from 0.85μg/g to 195.3μg/g in the landraces. Highest iron containing rice was Swetonunia with 34.8μg/g and highest Zn was found in Nepali Kalam which was 195.3μg/g. Zn content is quite comparable to other HYVs and can be used as donor parent in breeding program for biofortification of micronutrient Zn.
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    ItemOpen Access
    Screening of zinc resistant bacteria isolated from coal mine overburden soil
    (University of North Bengal, 2013-03) Tapadar, S.A.; Jha, D.K.
    Heavy metal pollution of coal mine spoils is a significant environmental problem affecting both flora and fauna. These degraded soils can be remediated by using heavy metal resistant microorganisms. Zinc has been usually reported in high concentration from coal mine spoils. The present study, therefore, deals with screening of Zinc, resistant bacteria isolated from coal mine spoils. The bacterial isolates were isolated from coal mine spoils of Tinsukia district of Assam. For the isolation of bacteria, King's B Medium and Pikovskaya's Agar Media were used. Heavy metal resistance of the bacterial isolates against zinc was tested on nutrient agar and sucrose-minimal salt low phosphate (SLP) medium. The results showed that out of the 20 test isolates 11 isolates were resistant. The minimum inhibitory concentration (MIC) ranged from 0.001 M to 0.02 M. Antibiotic resistance was studied for two most resistant isolates. The optimal growth conditions with reference to pH and temperature of the two most resistant bacterial isolates were evaluated. Growth pattern of the most resistant isolates was determined in different concentrations of zinc amended broth media. On the basis of cultural and biochemical characters, the resistant isolates were identified as Pseudomonas sp, Bacillus sp and Rhizobium sp. Some of these isolates also exhibited plant growth promoting (PGP) traits and phosphate solubilizing ability.
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    ItemOpen Access
    Responses of Zn and Cd treatment in soybean and fenugreek
    (University of North Bengal, 2012-03) Aery, NC; Sarkar, S
    The effect of various doses of' zinc and cadmium on morpho-anatomical and physiological parameters of soybean and fenugreek was studied. High concentrations of both Zn as well as Cd resulted in chlorosis, necrosis, retardation of growth and reduction in leaflet size especially in soybean. A characteristic symptom was the formation of a red-brown pigment in the leaves, stem and roots. An increase in the phenolic contents was also observed with increasing Cd doses. The reasons for the formation of pigment have been explored.