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Item Open Access Comparative study of basal thermo tolerant attributes in tolerant and susceptible wheat cultivars(University of North Bengal, 2019-03) Sarkar, Jayanwita; Chakraborty, Usha; Chakraborty, BishwanathTwo wheat cultivars, viz. C306 and HT41, have been evaluated for their responses to heat stress in terms of some biochemical and physiological attributes. During the exposure to high temperature (40°C for 6 and 12 h), a significant reduction in RWC and FW was recorded in HT41 which was also associated with heat susceptibility. The detrimental effects of high temperature were also apparent in terms of lipid peroxidation, chlorophyll content, H2O2 generation and electrolyte leakage and these were more pronounced in HT 41. Accumulation of osmolytes like proline, total sugar increased due to heat injury and also varied between the two cultivars. In this present study evaluation and analysis of these biochemical and physiological characters under heat stress could unravel the mechanism of basal thermotolerance and thus might be useful as genetic stock to develop wheat tolerant varieties.Item Open Access Evaluation of streptomyces and non-streptomyces actinomycetes isolates for growth promotion in Vigna radiata and their use as biocontrol agent against Sclerotium rolfsii(University of North Bengal, 2016-03) Ray, Pushpanjali; Chakraborty, Arka Pratim; Chakraborty, BishwanathTwo streptomyces (ARHS/PO26 and ARHS/PO27) and two non streptomyces (ARHS/Mn3 and ARHS/Mn7) actinomycetes isolates obtained from the rhizosphere soil of Solanum tuberosum and Mangifera indica were found to be phosphate solubilizers and showed antagonistic activity against Sclerotium rolfsii. Isolates ARHS/PO26 and ARHS/PO27 were identified morphologically and confirmed by the National Centre for Fungal Taxonomy, as Streptomyces griseus (NCFT 2578.08; NAIMCC-B-00916) and Streptomyces griseolus (NCFT 2579.08). ARHS/Mn 3 and Streptomyces griseolus (ARHS/PO27) could inhibit 68% and 59.7% growth of Sclerotium rolfsii in vitro. In vivo evaluation of the isolates ARHS/Mn 3, Streptomyces griseolus (ARHS/PO27) and Streptomyces griseus (ARHS/PO26) showed maximum growth promotion on Vigna radiata by enhancing key defense enzymes like chitinase, β-1,3-glucanase, phenylalanine ammonia lyase and peroxidase. The results emphasize the fact that soil actinomycetes could be used as potential biocontrol agents.Item Open Access Integrated management of seedling blight disease of tea caused by Sclerotium rolfsi(University of North Bengal, 2015-03) Bhagat, Indramani; Chakraborty, BishwanathTea is one of the important plantation crops in Nepal. One of the important fungal pathogens Sclerotium rolfsii, causing seedling blight disease in tea was found to be predominant in the nursery grown plants. The art and science of plant disease control has moved in the direction of biological control of plant pathogen is a distinct possibility for the future and can be successfully exploited in modern agriculture, especially within the framework of integrated disease management systems. Effective integrated management practices against S. rolfsii were developed using neem cake, oil cake, aqueous leaf extract of Azadirachta indica, bio-control agent like Trichoderma harzianum and calixin (0.1 vi%) in vivo. Combination with cow dung, neem cake, oil cake, chicken manure and rabbit manure, disease reduction were insignificant. However, combination with neem cake and oil cake showed 66.4% disease incidence, whereas in oil cake, neem cake and Azadirachta indica in combination disease incidence were recorded 11.1%. Under pot culture conditions T. harzianum alone and in combination with neem cake, oil cake and Azadirachta indica provided best effective management practices of seedling blight in all the three modes of application viz., simultaneous, repeated and post infection.Item Open Access Morphological characterization of rice cultivars their root colonization with arbuscular mycorrhizal fungi and screening for field resistance caused by brown spot disease(University of North Bengal, 2015-03) Khati, Sweata; Chakraborty, BishwanathVariability in seed morphology was studied in 15 rice cultivars using qualitative and quantitative characters. Germplasm of these indigenous rice landraces were collected from Bijanbari, Kalimpong, Sikkim, Malda, Siliguri and UBKV (Uttar Banga Krishi Viswavidyalaya). Data were recorded for traits such as Kernel colour, Seed cóat colour, Aroma, Presence of Awn and Length of the seed. A total of 9 landraces had white kernel colour while 4 had brown and 2 had greyed-orange. The seed coat colour variation in different landraces ranged from Golden yellow, Yellow, Red and Black. 6 landraces were having aroma whereas 9 had no aroma and lastly 11 landraces were found to have awn and 4 were awnless. UBKV-4 was longest in length with 1.1 cm and Sano masuri being the smallest of 0.4 cm. Establishment of disease in naturally infected rice cultivars were observed and disease index was calculated. Arbuscular Mycorrhizal Fungi (AMF) were screened from rhizosphere of fifteen rice cultivars grown on experimental field using wet sieving and decanting method. Microscopical observation revealed the presence of different genus of sp. Were found to high in all the fifteen cultivars of rice plants followed by Gigaspora, Scutellospora and Acaulospora. Histopathological study of roots showed the presence of vesicles and arbuscules. AMF infection and total number of spores per 100 grams of soil were recorded. Present study evaluates the study of different AMF population and their histopathology harbouring in the rhizosphere of rice.Item Open Access Development of an easy and efficient technique for cultivation of different species of Pleurotus(University of North Bengal, 2014-03) Roy, Somnath; Barman, Shibu; Chakraborty, Usha; Chakraborty, BishwanathOyster mushroom is one of the most popular mushrooms in North Bengal and a large number of growers are now cultivating oyster mushroom throughout the year. There are mainly three species of oyster mushroom (Pleurotus ostreatus, P. sajor-caju, P florida) which are being cultivated in this region. Out of these three mushrooms, P. ostreatus and P. sajor-caju are generally cultivated in summer as it grows in a temperature between 25-33C while P. florida is generally cultivated during winter as it requires 15-20°C for its growth. Bottle cultivation is a technique of mushroom cultivation where it reduces the cost of plastic bags and the plastic bottles can be recycled. Besides plastic bottles, other used laboratory chemical plastic containers were also tested and gave good results.