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Item Open Access Morphological and Biochemical Characterization of Novel Cellulose and Starch Degrading Bacteria Isolated from the Rhizospheric Soil of Dendrocalamus minor and Musa sp.(University of North Bengal, 2023) Chettri, Neha; Singh, Rishika; Misra, Megha; Sarkar, Sulagna; Toppo, Prabha; Bhandari, Jnan Bikash; Mathur, PiyushThe rhizosphere is the most active region of soil where plants and microorganisms live in close association and exhibit complex interactions. In the present study, rhizospheric soil samples were collected from bamboo and banana growing well in University of North Bengal campus. Cellulolytic and amylolytic bacteria were isolated from these samples through serial dilution technique and were identified on the basis of morphological and biochemical characteristics. The study showed presence of four isolates mostly from genera Bacillus sp. from rhizosphere of bamboo capable of degrading cellulose while five isolates (Rummeliibacillus sp., Lysinibacillus, Brevibacillus, and Bacillus) from rhizosphere of banana and degrade starch respectively. The study although preliminary but will prove valuable for the extraction of these enzymes from these rhizospheric isolates and will be highly appreciated for their application in biotechnology sector.Item Open Access Study of Major Isoflavones in Mungbean Seedlings with Special Emphasis on Its Enhanced Antioxidant Activity After Solid Matrix Priming with Selected Elicitors Including Nano-Chitosan Under Salinity Stress(University of North Bengal, 2022) Sen, Sujoy Kumar; Mandal, Palash; Bhandari, Jnan BikashFor a long time, mung bean has been a well-liked crop. It is frequently used as a popular dish in the primarily cereal-based diets of Asian countries for its physiological functionalities, such as antioxidant, antitumor, and antidiabetic activities. Isoflavones present in legume-based foods have high antioxidant potential. These isoflavones are considered beneficial to human health and are linked to a reduced risk of cardiovascular disease, osteoporosis, and the prevention of certain types of cancer in humans, including breast, prostate, and colon cancer, as well as menopausal symptoms. On the other hand, nanotechnology is starting to look like an excellent method to boost food production and make farming less hazardous to the environment. Fascinatingly, the seed nano-priming method demonstrated promising results to mitigate the detrimental effects of different abiotic stress factors including salinity stress on crop plants and has thus, led to higher crop yields. The current study aimed to evaluate the effects of solid matrix priming (SMP) using nano-chitosan in mung bean sprouts under salinity stress related to the production of major mung bean isoflavones, which were detected through high-resolution liquid chromatography-mass spectrometry. When compared to unprimed seedlings exposed to salinity stress conditions, phytochemical quantification showed that SMP with nano-chitosan showed improved antioxidant activities as well as the highest total flavonoids and proline content. Under salinity stress, SMP with nano-chitosan significantly increased the biochemical anti-oxidative properties in germinated mung bean seeds, and also provided salt tolerance. As a familiar healthier choice, and because of the significance of mung bean sprouts for human health and the industry's rapid expansion, nutritional enrichment of this food has emerged as a significant field of study.Item Open Access Taxonomic Investigation of Some Members of Fabaceae (Subfamily- Caesalpinioidae) With Special Reference to Pollen Morphology(University of North Bengal, 2020-03) Mondal, Swarnaditya; Roy, Biswajit; Bhandari, Jnan BikashPalynology, the study of pollen, had a big role in taxonomic identification, paleontology and forensics. Pollens from different plants had different morphology, such as in Fabaceae. This study aimed to determine the pollen grains morphology in Fabaceae (Subfamily Caesalpinioidae). Pollen morphology of 19 plant species under 7 genera Fabaceae (Subfamily Caesalpinioidae) were examined. Fresh pollen samples were collected from 4 plant species from the North 24 Pgns, 2 plant species from South 24 Pgns, 8 plant species from Kolkata, 2 plant species from Nadia and 3 plant species from Howrah. Pollens were acetolysed following standard method and observed under Compound microscope. Pollen shapes were recorded based on the P/E ratio. In general, all these pollens were small, medium and large size. Parameters measured in this study were the types of pollen sizes, pollen shape, aperture characteristic, and ornamentation type of exine and the most common aperture type was tricolporate. The most important characters included exine ornamentation (exine ornamentation type) and Apocolpium Index.