NBU Journal of Plant Sciences

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The NBU Journal of Plant Sciences serves as the official organ of the Department of Botany for the publication of research papers by its members and researchers. The journal is sent free to its members and different academic institutions on demand.

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Author: search.filters.author.Chakraborty, P.Subject: search.filters.subject.Begomovirus

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    Prevalence of begomoviruses associated with tomato leaf curl disease in the sub-Himalayan plains of West Bengal
    (University of North Bengal, 2016-03) Karmakar, A.; Chakraborty, P.; Sarkar, T.; Das, S.; Saha, A.; Saha, D.; Saha, A.
    Tomato is a solanaceous crop and one of the most economically important vegetables in the world. India ranks second in total production of tomato in the world. It has been referred to as a “functional food”, a food that goes beyond providing just basic nutrient. To LCD is one of the major constraints to tomato production in India. To study the disease incidence of tomato, a survey was made in the tomato crop growing fields of Darjeeling, Jalpaiguri, Coochbehar and Uttar Dinajpur districts of sub-Himalayan West Bengal during December 2015 to February 2016 and several infected and healthy leaf samples were collected based on the morphological symptoms like-vein cleaning, leaf curling, leaf deformations and stunted growth of plants. Disease incidence ranged from 70% to 86.66% of the collected samples from different districts. All the samples collected from the present study area were tested by PCR with DengA and DengB primer and expected amplicon of ̴ 530bp was found. Two randomly selected PCR positive samples were sequence and analyzed (Acc. Nos KX108859 and KX108860). The SLG-1 isolate (Acc. No. KX108859) showed 95 % nt identity with ToLCKV (Acc. No. KP178730) and the ISL-1 isolate (Acc. No. KX108860) showed 96% nt identity with ToLCNDV (Acc. No. KC513822). The threat of begomoviral spread to the north-eastern part of India has been taken into consideration.