Prevalence of begomoviruses associated with tomato leaf curl disease in the sub-Himalayan plains of West Bengal
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Type
Article
Date
2016-03
Journal Title
NBU Journal of Plant Sciences
Journal Editor
Chakraborty, Usha
Journal ISSN
Volume Title
Publisher
University of North Bengal
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Karmakar, A., Chakraborty, P., Sarkar, T., Das, S., Saha, A., Saha, D., & Saha, A. (2016). Prevalence of begomoviruses associated with tomato leaf curl disease in the sub-Himalayan plains of West Bengal. NBU Journal of Plant Sciences, 10(1), 66–72. https://ir.nbu.ac.in/handle/123456789/4533
Authors
Karmakar, A.
Chakraborty, P.
Sarkar, T.
Das, S.
Saha, A.
Saha, D.
Saha, A.
Advisor
Editor
Abstract
Tomato is a solanaceous crop and one of the most economically important vegetables in the world. India ranks second in total production of tomato in the world. It has been referred to as a “functional food”, a food that goes beyond providing just basic nutrient. To LCD is one of the major constraints to tomato production in India. To study the disease incidence of tomato, a survey was made in the tomato crop growing fields of Darjeeling, Jalpaiguri, Coochbehar and Uttar Dinajpur districts of sub-Himalayan West Bengal during December 2015 to February 2016 and several infected and healthy leaf samples were collected based on the morphological symptoms like-vein cleaning, leaf curling, leaf deformations and stunted growth of plants. Disease incidence ranged from 70% to 86.66% of the collected samples from different districts. All the samples collected from the present study area were tested by PCR with DengA and DengB primer and expected amplicon of ̴ 530bp was found. Two randomly selected PCR positive samples were sequence and analyzed (Acc. Nos KX108859 and KX108860). The SLG-1 isolate (Acc. No. KX108859) showed 95 % nt identity with ToLCKV (Acc. No. KP178730) and the ISL-1 isolate (Acc. No. KX108860) showed 96% nt identity with ToLCNDV (Acc. No. KC513822). The threat of begomoviral spread to the north-eastern part of India has been taken into consideration.
Description
Citation
Accession No
Call No
Book Title
Edition
Volume
ISBN No
Volume Number
10
Issue Number
1
ISSN No
0974-6927
eISSN No
Pages
Pages
66 - 72