NBU Journal of Plant Sciences, Vol. 05, No. 01
Permanent URI for this collectionhttps://ir.nbu.ac.in/handle/123456789/4434
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Item Open Access Phytochemical screening and antibacterial activity of Plagiochasma appendiculatum Lehm. et Lindenb against pathogenic bacteria(University Of North Bengal, 2011-03) Khanam, Rubina; Chaudhary, BL; Khanam, SThe in vitro antibacterial activity of whole thallus of Plagiochasma appendiculatum and its fractions petroleum ether, benzene, acetone, methanol, ethanol, and aqueous extracts were tested against the growth of four human pathogenic gram negative bacteria namely Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae and two gram positive bacteria Bacillus subtilis and Staphylococcus aureus using agar well diffusion technique. The plant showed significant antibacterial activity against almost all the organisms. The maximum antibacterial activity was observed in methanolic extract against Escherichia coli and minimum activity was observed in petroleum ether extract against Staphylococcus aureus. The phytochemical analysis of the extract indicated the presence of saponin, flavonoids, and sesquiterpenes. The inhibitory effect of the extract was compared with standard antibiotics, streptomycin.Item Open Access Sea buckthorn and its microsymbiont-a review(University Of North Bengal, 2011-03) Basistha, Bharat C; Sen, ArnabHippophae sp. is a versatile plant restricted in distribution to the Himalayas having multipurpose usage including food, fodder, medicine, and controlling soil erosion. Besides, it plays a huge role in increasing the fertility of the soil by harboring symbiotic nitrogen fixing bacteria called Frankia. In this review we have looked into two main aspects of this symbiosis. First we have made a detailed account of the macrosymbiont i.e. Hippophae. Since Hippophae has food and medicinal properties and are widely used in cosmetic production, we excavated the antioxidant activity of various parts of Hippophae including fruits, seeds, bark and leaf. People of Indo-Tibetan plateau adapt a special agro-technique to cultivate Hippophae. The technique has been discussed here. A detail report of this plant including their distribution and various ecological parameters has also been done. On the other hand we have also elucidate about the microsymbiont present in root nodule of Hippophae i.e. Frankia. Frankia is filamentous actinomycetes which fix atmospheric nitrogen to the soil and therefore increase the soil-fertility. A detailed account of morphology. anatomy, phylogeny and ecology of Frankia has been illustrated here. The diversity of Frankia in soil is another interesting topic and the speciation of this bacterium is an everlasting controversy. We have given a closer look to the genetic diversity and phylogenetic relationships of Frankia at intra and inter generic level.Item Open Access An ethno-medicobotanical investigation among Rava tribe of Jalpaiguri district(University Of North Bengal, 2011-03) Bose, DebadinThis work deals with the ethnomedicobotanical use of various plant species by Rava community residing in Jalpaiguri district. This kind of approach is rather sporadic not only in this region but also to other parts of the India. Here ethnomedicinal uses of 41 selected plant species, which are generally used by the Rava people, have been described. This study reveals some interesting information about the mode of the use ot these plants which may form the basis of primary health care system of Rava community. This knowledge would be an excellent addition to the current knowledge of folk medicine and may opens a new horizon in the field of ethnobotany and ethnomedicine.Item Open Access Domestication of some wild edible plants in Barail range of Assam: a case study(University Of North Bengal, 2011-03) Nath, KK; Deka, P; Nath, D; Borthakur, SKAboriginal societies have their own approaches in managing their resources. Assam is one of the states of Northeast India with both biological and ethnic diversity. Generally aboriginal societies have been living in complete harmony with natural surroundings. They have their own notions, beliefs and way of sustainable utilization and conserving the plant resources. They are the custodians of much useful information regarding plant resources in the form of oral folklore. The present communication deals with 24 little known wild plant species, which have been conserving in the vicinity of the villages through domestication and/or restricted cultivation for their tubers, stems, leaves, inflorescences or fruits by the Dimasa. The Hmar, the Zeme Naga and the Kuki tribes of Barail Range in Dima Hasao (North Cachar Hills) district of Assam. The initiatives of the ethnic groups of the area are very significant from conservation point of view especially of wild plant genetic resources.Item Open Access Effect of cerium on seed germination and early seedling growth of wheat(University Of North Bengal, 2011-03) Shyam, Radhe; Aery, NCThe effect of various concentrations of three species of cerium was studied on seed germination and early seedling growth of Triticum aestivum L. Seeds were germinated on moist filter papers added with 0.1, 0.5, 2.5, 12.5 and 62.5 Hg g' cerium as cerium chloride, cerium sulphate and cerium nitrate and observations were made on seed germination, shoot- root length, fresh and dry weight. Lower concentrations of cerium species significantly increased shoot-root growth and relative yield of seedlings. Higher concentrations (12.5 and 62.5 Hg g) proved to be toxic for seedling growth. The root growth was affected more adversely than shoots. Though germination speed was affected, ultimate germination was always 100 %. The results of this study indicate that low levels/ concentrations of cerium are beneficial for the test plants.Item Open Access A simple method for large scale estimation of leaf chlorophyll content in Hevea brasiliensis using a chlorophyll meter(University Of North Bengal, 2011-03) Nair, DB; Jacob, JamesMeasurement of chlorophyll content of leaves by the conventional laboratory method involves discriminate sampling. weighing. extraction and spectrophotometric measurements that become laborious and time consuming especially when large number of samples is processed at a time. Nowadays, hand-held chlorophyll content meters are widely used for rapid and non-destructive estimation of chlorophyll content. Chlorophyll content in Hevea leaves was estimated spectrophotometrically by conventional extraction method and CCI values (chlorophyll content index) were recorded using a chlorophyll content meter, CCM - 200 (Opti-Sciences, USA) and regression models were developed. Significant positive linear relations between CCI and actual leaf chlorophyll content estimated by conventional extraction method were obtained. Separate equations were derived for determining chlorophyll content on unit fresh weight basis and unit leaf area basis. The method provides a rapid, accurate and non-destructive estimation of chlorophyll content of large number of leaves making the measurements simple and easy compared to conventional spectrophotometric assay.Item Open Access Free radical scavenging activity of ornamental and edible cultivars of Canna found in Eastern India(University Of North Bengal, 2011-03) Mishra, T; Goyal, AK; Mondal, P; Sen, ACanna, the only genus of family Cannaceae, found in eastern India have gained economic importance for their starchy rhizomes and attractive flowers. The rhizome of some edible cultivars are consumed as food and herbal medicines in rural areas. The presence of total phenols, flavonoids and DPPH scavenging activity of both cold and hot extracts and their correlations have been studied for eleven cultivars of Canna spp. Antioxidants, quercetin and gallic acid equivalence of phenolic compounds were determined spectrophotometrically. It has been found that total phenol, flavonoids and DPPH free radical scavenging activity were higher in hot extracts than in cold. Further studies gave an idea about the correlation between phenol and flavonoid contents of both cold and hot extracts, but both the above said parameters were not correlated with DPPH scavenging activity. Finally it can be concluded that higher DPPH scavenging activities in all these Canna cultivars may not be due to the presence of endogenous phenols and flavonoids but these may be due to any other phytochemicals.Item Open Access Assessment of genetic diversity of some commonly grown rice genotypes of South Bengal using microsatellite markers associated with the saltol QTL mapped on 1"chromosome(University of North Bengal, 2011-03) Lodha, T; Karmakar, J; Roychoudhuri, R; Dey, NA total of 16 common rice lines and one wild rice relative grown in rarh south Bengal were genotyped using six polymorphic microsatellite markers associated with saltol QTL mapped on rice chromosome. DNA fingerprint profiles identified each of the 17 rice genotypes unequivocally and the pair-wise polymorphism data for the studied genotypes were used to analyze the genetic diversity present within the studied rice lines. The number of alleles per SSLP marker, the size range of the PCR products and the polymorphism information content (PIC) values of each marker were calculated out. A dendrogram was constructed using the average linkage (within group) and similarity coefficient among the studied genotypes which indicated that a considerable amount of genetic diversity is present and classify the studied genotypes into two major clusters and one minor cluster.Item Open Access Plant wealth of Darjiling and Sikkim Himalayas vis-à-vis conservation(University of North Bengal, 2022-03) Das, AP; Ghosh, ChandraThe richness of the flora of Darjiling Hills along with Sikkim and Nepal parts of the Eastern Himalaya is well known and that has attracted plant lovers, explorers and hunters almost equally for the last three centuries or so. The processes of evolution worked in this part of the Himalayas almost in an undisturbed condition for millions of years assisted by much varied but extremely favourable climatic conditions and has produced innumerable new species, a good proportion of which are still endemic to the region. The vegetation structure and the flora in different parts of this region have developed as per the local climatic make-up. So, when almost tropical vegetation is found on outer low altitude areas, it is sub-alpine to alpine to arctic in high altitude areas especially above 3000 m. Terai and Duars are located at the feet of the hills are maintaining a contiguous rich vegetation. The vegetation scenario in this region is changing very fast during last three or four decades mostly due to anthropogenic activities. Extension of civilisation along with the population explosion lead to the increase in forest extraction, rate of modification of floristic composition, pollution content, soil erosion, plant introduction, clearing of natural vegetation etc. There is evidence that the force of evolution is still active here but the lack of proper corridor (along with other factors mentioned above) for plant migration will certainly cause the weakening of numerous local species and the rate of extinction of species will increase in a logarithmic scale. Like any other part of this planet here also it appears to be one nearly impossible task to save the local biodiversity. Just the declaration of some Protected Areas is not enough. Activities through ecotourism are also affecting the conservation practices. Unless we succeed to conserve the natural vegetation on this planet the entire biosphere will be dead within next 100 years.Item Open Access Enterobacter nickelidurans sp. nov., a novel nickel tolerant enterobacteria isolated from Torsa river water of India(University of North Bengal, 2011-03) Bhadra, Bhaskar; Nanda, Ashis Kumar; Chakraborty, RanadhirA Gram-negative, rod-shaped, nickel-resistant bacterium, designated as strain NiVas 114', was isolated from waters of Torsa River in Hasimara, West Bengal, India. The strain NiVasl 14 possessing inducible nickel resistance can tolerate maximally 10mM nickel chloride. Southern blot assays of genomic DNA of NiVas 114 using probe(s) generated from known nickel resistance determinants (cnr/ ncc/ nrel nerí nir). under conditions of low stringency, produced no detectable signal except for cnrA gene of Ralstonia metallidurans CH34 (formerly Alcaligenes eutropha CH34) in which weak hybridization signal occurred. Based on 16S rRNA gene sequence similarity, strain NiVas 114 was identified as a member of y Proteobacteria, and the nearest phylogenetic relatives are Enterobacter hormaechei (98.6-98.5%) and Enterobacter cloacae (97.5-98.2 %). In the phylogenetic trees constructed with nucleotide sequence of 16S rRNA gene, hsp60 gene and rpoB gene, strain NiVas 114" clustered with the subspecies of Enterobacter hormaechei. The strain showed DNA-DNA relatedness of 60 -63% with Enterobacter hormaechei subsp hormaechei, Enterobacter hormaechei subsp. oharae, Enterobacter hormaechei subsp. steigerwalti Enterobacter cloacae subsp. cloacae and Enterobacter cloacae subsp. dissobvens. The isolate NiVas 114 differed from the nearest phylogenetic relatives in terms of number of phenotypic characteristics. The G+C content of the genomic DNA of the isolate was 59.5 +0.4 mol %. The predominant cellular faty acids of the isolate are C160, Summed feature 3 (comprises C16wTel 15 io 201) and C 18 w7ei hydroxy fatty acids are found in minor quantities. Thus, on the basis of biochemical characteristics, fatty acid profiles, DNA-DNA relatedness and phylogenetic analysis, the isolate was recognized as a novel species of Enterobacter. for which the name Enterobacter nickellidurans sp. nov. is proposed with the type strain NiVas 114' (= LMG 23000 = CcUGSO594"= JCM13045").