Enterobacter nickelidurans sp. nov., a novel nickel tolerant enterobacteria isolated from Torsa river water of India
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Type
Article
Date
2011-03
Journal Title
NBU Journal of Plant Sciences
Journal Editor
Sen, Arnab
Journal ISSN
Volume Title
Publisher
University of North Bengal
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Bhadra, B., Nanda, A. K., & Chakraborty, R. (2011). Enterobacter nickelidurans sp. nov., a novel nickel tolerant enterobacteria isolated from Torsa river water of India. NBU Journal of Plant Sciences, 5(1), 15–23. https://ir.nbu.ac.in/handle/123456789/4437
Authors
Bhadra, Bhaskar
Nanda, Ashis Kumar
Chakraborty, Ranadhir
Advisor
Editor
Abstract
A Gram-negative, rod-shaped, nickel-resistant bacterium, designated as strain NiVas 114', was isolated
from waters of Torsa River in Hasimara, West Bengal, India. The strain NiVasl 14 possessing inducible
nickel resistance can tolerate maximally 10mM nickel chloride. Southern blot assays of genomic DNA of
NiVas 114 using probe(s) generated from known nickel resistance determinants (cnr/ ncc/ nrel nerí nir).
under conditions of low stringency, produced no detectable signal except for cnrA gene of Ralstonia
metallidurans CH34 (formerly Alcaligenes eutropha CH34) in which weak hybridization signal occurred.
Based on 16S rRNA gene sequence similarity, strain NiVas 114 was identified as a member of y
Proteobacteria, and the nearest phylogenetic relatives are Enterobacter hormaechei (98.6-98.5%) and
Enterobacter cloacae (97.5-98.2 %). In the phylogenetic trees constructed with nucleotide sequence of 16S
rRNA gene, hsp60 gene and rpoB gene, strain NiVas 114" clustered with the subspecies of Enterobacter
hormaechei. The strain showed DNA-DNA relatedness of 60 -63% with Enterobacter hormaechei subsp
hormaechei, Enterobacter hormaechei subsp. oharae, Enterobacter hormaechei subsp. steigerwalti
Enterobacter cloacae subsp. cloacae and Enterobacter cloacae subsp. dissobvens. The isolate NiVas 114
differed from the nearest phylogenetic relatives in terms of number of phenotypic characteristics. The G+C
content of the genomic DNA of the isolate was 59.5 +0.4 mol %. The predominant cellular faty acids of
the isolate are C160, Summed feature 3 (comprises C16wTel 15 io 201) and C 18 w7ei hydroxy fatty acids are
found in minor quantities. Thus, on the basis of biochemical characteristics, fatty acid profiles, DNA-DNA
relatedness and phylogenetic analysis, the isolate was recognized as a novel species of Enterobacter. for
which the name Enterobacter nickellidurans sp. nov. is proposed with the type strain NiVas 114' (= LMG
23000 = CcUGSO594"= JCM13045").
Description
Citation
Accession No
Call No
Book Title
Edition
Volume
ISBN No
Volume Number
5
Issue Number
1
ISSN No
0974-6927
eISSN No
Pages
Pages
15 - 23