NBU Journal of Plant Sciences, Vol. 09, No. 01

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    Screening of free-living bacteria from the rhizosphere of Jute for their multiple plant growth promoting and antagonistic activity against phytopathogens
    (University of North Bengal, 2015-03) Majumdar, Sukanta; Chakraborty, Usha
    Present investigation was undertaken to screen the PGPR isolates from the rhizosphere of Jute for their plant growth promoting and antagonistic activities in the view of an alternative way to chemical fertilizer and hazardous fungicides. A total 76 isolates were isolated from different parts of northern West Bengal and screened for their antagonistic activity against Macrophomiona phaseolina. Most promising five isolates were selected for further study and screened for other plant growth promoting and lytic enzyme producing abilities. Out of these, two isolates were Gram positive and rest three Gram negative. All five isolates exhibited several plant growth promoting activities. All five isolates showed IAA and ammonia production whereas four out five showed phosphate solubilization activity. Three PGPR strains exhibited siderophore production and only one isolate showed cyanide production ability. Among the lytic enzymes, chitinase was produced by three isolates. Among them B-3 showed highest degree of chitinase production. Protease was also produced by four strains but amylase and B-1,4-glucanase activity showed by only one isolate, Act-6. Two isolates B-3 and Acti-6 showed considerable amount of antagonistic activity against three phytopathogens Macrophomina phaseolina, Fusarium oysporum, and F. semitectum suggesting that Acti-6 and B-3 showed several attributes to be the potent strains of PGPR and can be used as biofertilizer as well as biocontrol agents.
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    Management of grey blight disease of Som plants using value added vermicompost with Glomus constrictum and Bacillus altitudinis
    (University of North Bengal, 2015-03) Acharya, A.; Chakraborty, U.; Ghosh, S.; Chakraborty, B.N.
    Grey blight disease caused by Pestalotiopsis disseminata, is one of the major foliar fungal diseases that constantly affects Persea bombycina Kost, a primary host plant of muga silkworm. Under nursery condition, grey blight disease was recorded mostly in SS and S6 morphotypes of som plants. Vermicompost, PGPR and AMF, alone and in combination were applied for the improvement of the growth of eight morphotypes of som plant as well as to reduce incidence. Growth in terms of height (cm), no. of leaves and no. of branches were studied. Analysis of some major defence related enzymes such as POX, PAL, CHT and GLU was also carried out to check induction of resistance after treatment. Artificial inoculation of som plants under nursery condition with spore suspension of P. disseminata was performed and disease progression noted for 7, 14, 21 and 28 days. It was clearly seen that disease progression was slow and less in treated inoculated plants. The results emphasize the fact that application of bioinoculants can be studied in larger scale for the upliftment of the health status of muga host plants.