Role of germination induced peptide pool in plant tissue culture
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Type
Article
Date
2009-03
Journal Title
NBU Journal of Plant Sciences
Journal Editor
Chakraborty, U
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Volume Title
Publisher
University of North Bengal
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Mandal, P., Misra, T., Ghosh, A., & Sircar, P. (2009). Role of germination induced peptide pool in plant tissue culture. NBU Journal of Plant Sciences, 3, 53–58. https://ir.nbu.ac.in/handle/123456789/4416
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Abstract
Low molecular weight peptides (ranges from 3.0 KDa to 0.5 KDa) were extracted and purified from rice, wheat, chickpea and mungbean through cryocrushing, cold centrifugation, ether fractionation, cation and anion-exchange column chromatographic separation, lyophilisation and ultrafiltration. The profile of heterogeneous peptides was detected through one dimensional paper chromatography. Peptüde fractions isolated from different germination hours of mungbean enhanced mitotic index, more particularly prophase of root tip of Alium cepa L and excuted definite control over morphogenesis of excised and cotyledonary embryo culture of chick-pea seeds (Cicer arietinum L.). Restricted callogenesis in carrot pith culture was observed by the application of wheat peptides without any other hormones. Our speculation is that the peptides can mimic the action of hormone and behave as novel kind of bioactive molecule through which the physiological responses can be modulated.
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Volume Number
3
Issue Number
ISSN No
0974-6927
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Pages
Pages
53 - 58