NBU Journal of Plant Sciences
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The NBU Journal of Plant Sciences serves as the official organ of the Department of Botany for the publication of research papers by its members and researchers. The journal is sent free to its members and different academic institutions on demand.
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Item Open Access A conference report on 19th International Meeting on Frankia and Actinorhizal Plants(University of North Bengal, 2019-03) Sarkar, IndraniThe 19th International conference on Frankia and Actinorhizal plants which I attended along with one of my colleague Miss Reha Labar was held from 17th-10th March, 2018 at Hammamet, Tunisia Since my research is about Frankia and other actinobacteria, this conference provided a full opportunity to meet with people from different parts of the world who are working on the same topic and also learned some new techniques they are using for better understanding of Frankia and Actinorhizal symbiosis.Item Open Access Effect of solvents on qualitative and quantitative phytochemical constituent profiles of fresh leaves of TV26(University of North Bengal, 2019-03) Labar, Reha; Sen, Armab; Bhattacharya, MalayThe aim of this study was to qualitatively and quantitatively screen and identify major phytochemical groups from leaves extract of TV26 extracted by nine different solvents having different polarities. Qualitative screening suggested acetone, methanol, ethanol and ethyl acetate to be the most potent solvent for various phytochemical extractions like flavonoid, tannin, steroid, diterpenes, terpenoids, coumarin, cardiac glycoside, saponin, protein and reducing sugar. The highest percent of radical scavenging was recorded in cold water extracts (5mg/ml) i.e. 91.10% and was at par with 93.40% percent scavenging activity of ascorbic acid taken as standard (5mg/ml). Methanol, acetone, ethanol and ethyl acetate gave the best results with the total phenol content value (GAE) recorded as 100.60mg/g, 87.07mg/g, 58.73 mg/g and 51.47mg/g respectively with methanol giving the best result. Acetone extracts (5mg/ml) showed higher ferric reducing power with IC, value 426.45+ 1.12 μg/ml compared to the standard (ascorbic acid) 270.35-0.66 μg/ml. Our findings suggest that the polar solvents were more beneficial and potent against the other non polar counterparts during phytochemical extraction but the polarity of solvents need not be in increasing order since we can assume from our results that acetone being less polar than ethanol, methanol and water showed better results. In addition to different polarities, state of the sample and extraction technique is also crucial for better extraction.Item Open Access RAPD analysis and rDNA gene sequence based phylogeny of Bipolaris sorokiniana, a spot blotch pathogen of sorghum(University of North Bengal, 2019-03) Bhattacharjee, Priyanka; Sen, Armab; Chakraborty, Usha; Chakraborty, BiswanathSorghum [Sorghum bicolor (L) Moench] is the one of the most important cereal crops in the world. It is the staple food grain for over 750 million people who live in the semi-arid tropics of Africa, Asia, and Latin America. Global pro-duction of sorghum is currently estimated to be 57.6 million tonnes, with Asian countries contributing 20% of the total production. Within Asia, India is the largest producer of sorghum grain. Recently there have been severe signs of sorghum decline caused by Bipolaris sorokiniana resulting in decreased production of sorghum in villages of Kalimpong and Darjeeling. In the present study, initially, several strains of the fungus were isolated from diseased leaves of Sorghum bicolor and Triticum aestivum which were morphologically identified as Bipolaris sorokiniana. Genomic DNA of B. sorokiniana isolated from infected leaves was purified and PCR amplification of 18s rDNA was done using specific primers. Amplified product (1190 bp) was sequenced and aligned against ex-type strain sequences of B. sorokiniana from NCBI GenBank using BLAST and phylogenetic analysis was done using MEGA4 software. RAPD PCR analysis and DGGE analysis of amplified genomic DNA were done. The evolutionary history was inferred using the UPGMA method. Amplification of ITS region of the rDNA can be considered as a rapid technique for identifying pathogens successfully in all cases.Item Open Access Pharmacological Activity of Citrus macroptera: an overview of a medicinal plant(University of North Bengal, 2019-03) Lala, Mousikha; Bhattachary, Malay; Sen, ArnabPlants and plant parts have been extensively used as medicine from the dates of early civilization. They are low in cost and have least side effects. In India, the practice of the traditional medicinal can be traced back to 5000 B.C. Citrus macroptera (Mont.) of the family Rutaceae is a bitter fruit bearing plant commonly found in tropical and subtropical regions. It is an endangered wild plant of North-eastern India. Various parts of this plant such as leaves, bark, pulp, peel specially fruits have an immense range of medicinal importance and has been used in different kinds of ailments like fever, kidney and liver dysfunction, dyspepsia, cardiovascular disease, stroke and many more to mention. The fruit of this perennial spiny plant has potential antioxidant, cytotoxic, antimicrobial, antihypertensive, antipyretic and appetite stimulant activity. The principal phytochemical constituents of this plant are Ribalinine, Edulinine, Bergamottin, Psoralen, Marmin, Severine, Geipavarine, β-pinene, α-pinene, ρ-cimene, (E)-ocimene, vanilline, Rutin, kaempferol, sabinene, limonene, γ-terpinene and so forth. Furthurmore thrombolytic, antidiabetic, antidepressant, cardioprotective, hepatoprotective activity have been reported on this plant. This review represents a comprehensive study on the various aspects of Citrus macroptera with respect to their traditional and medicinal usage or better use as alternative medicine for various kinds of treatments.Item Open Access Conservation of germplasm and role of Centre for Conservation & Utilization of Medicinal and Aromatic Plant of NBU, West Bengal, India(University of North Bengal, 2019-03) Chowdhury, M.; Pal, A.; Mondal, S.; Paul, P.; Basak, M.; Chettri, D.; Chowdhury, R.Centre for Conservation & Utilization of Medicinal and Aromatic Plant is one of important Herbal garden in India and is located within the campus of University of North Bengal at the foothills of Darjeeling District, West Bengal. Present this ex-situ conservatories houses a total of 729 species of vascular plants belonging to 477 genera and 153 families. Several endemic and threatened species are growing in this garden. The garden is very good store house of various medicinal and aromatic plants and also involve in active research activity.Item Open Access Impact of invasive alien weeds on phytodiversity of Terai-Duars region of West Bengal, India(University of North Bengal, 2019-03) Biswas, Kishor; Das, Abhaya PrasadThe article reports the investigation on the impacts of alien invasive weeds on phyto-diversity of Terai-Duars belt of West Bengal. Invasive weed-invaded and non-invaded vegetation tracts, under same ecological conditions, were sampled and compared to understand their impact on the local biodiversity. Invaded area was inhabitated by a fewer number of species, poor in species diversity, with higher concentration of dominance and poor in species richness. The study suggested further research on the impact of aggressive and invasive alien weeds on Biodiversity and ecosystem of this area.Item Open Access Comparative study of basal thermo tolerant attributes in tolerant and susceptible wheat cultivars(University of North Bengal, 2019-03) Sarkar, Jayanwita; Chakraborty, Usha; Chakraborty, BishwanathTwo wheat cultivars, viz. C306 and HT41, have been evaluated for their responses to heat stress in terms of some biochemical and physiological attributes. During the exposure to high temperature (40°C for 6 and 12 h), a significant reduction in RWC and FW was recorded in HT41 which was also associated with heat susceptibility. The detrimental effects of high temperature were also apparent in terms of lipid peroxidation, chlorophyll content, H2O2 generation and electrolyte leakage and these were more pronounced in HT 41. Accumulation of osmolytes like proline, total sugar increased due to heat injury and also varied between the two cultivars. In this present study evaluation and analysis of these biochemical and physiological characters under heat stress could unravel the mechanism of basal thermotolerance and thus might be useful as genetic stock to develop wheat tolerant varieties.Item Open Access Management of Fusarium wilt of tomato caused by Fusarium oxysporum f sp. lycopersici(University of North Bengal, 2019-03) Bhagat, IndramaniFusarium wilt of tomato (Lycopersicum esculentum Miller) caused by Fusarium oxysporum f sp. lycopersici is one of the most destructive diseases in tomato throughout the world. Effective and efficient management of the crop disease is generally achieved by the use of synthetic pesticides. These pesticides cause deleterious effects on human health and biosphere. Amendments (Neem cake, oil cake, cow dung, rabbit manure and chicken manure) were used in tomato seedlings to observe growth promotion increase in healthy and treated tomato seedlings of two varieties, Shrijana and Patam. Results revealed that growth of the tomato seedlings was significantly increased following amendment with neem cake and oil cake in the treated Fusarium oxysporum f sp. lycopersici inoculated plants than in untreated uninoculated plants as recorded. Oil cake had better effect than neem cake manure. It has been observed that the growth of tomato seedlings increased in untreated inoculated than treated inoculated tomato seedlings. Among cow dung, rabbit manure and chicken manure, chicken manure gave better growth of tomato seedlings than that of rabbit manure and cow dung. Similarly effective integrated management practices against Fusarium oxysporum f sp. lycopersici were also developed using neem cake, oil cake, aqueous bulb extract of Allium sativum, bio-control agent like Trichoderma harzianum and calixin (0.0125%) in vivo. Combination with cow dung, neem cake, oil cake, chicken manure and rabbit manure, disease reduction were insignificant. However, combination with neem cake and oil cake showed 64.4% disease incidence, whereas in oil cake, neem cake and Allium sativum in combination disease incidence were recorded 10.1%. Under pot culture conditions T. harzianum alone and in combination with neem cake, oil cake and Allium sativum provided best effective management practices of Fusarium wilt in all the three modes of application viz., simultaneous, repeated and post infection.Item Open Access Evaluation of streptomyces and non-streptomyces actinomycetes isolates for growth promotion in Vigna radiata and their use as biocontrol agent against Sclerotium rolfsii(University of North Bengal, 2016-03) Ray, Pushpanjali; Chakraborty, Arka Pratim; Chakraborty, BishwanathTwo streptomyces (ARHS/PO26 and ARHS/PO27) and two non streptomyces (ARHS/Mn3 and ARHS/Mn7) actinomycetes isolates obtained from the rhizosphere soil of Solanum tuberosum and Mangifera indica were found to be phosphate solubilizers and showed antagonistic activity against Sclerotium rolfsii. Isolates ARHS/PO26 and ARHS/PO27 were identified morphologically and confirmed by the National Centre for Fungal Taxonomy, as Streptomyces griseus (NCFT 2578.08; NAIMCC-B-00916) and Streptomyces griseolus (NCFT 2579.08). ARHS/Mn 3 and Streptomyces griseolus (ARHS/PO27) could inhibit 68% and 59.7% growth of Sclerotium rolfsii in vitro. In vivo evaluation of the isolates ARHS/Mn 3, Streptomyces griseolus (ARHS/PO27) and Streptomyces griseus (ARHS/PO26) showed maximum growth promotion on Vigna radiata by enhancing key defense enzymes like chitinase, β-1,3-glucanase, phenylalanine ammonia lyase and peroxidase. The results emphasize the fact that soil actinomycetes could be used as potential biocontrol agents.Item Open Access Prevalence of begomoviruses associated with tomato leaf curl disease in the sub-Himalayan plains of West Bengal(University of North Bengal, 2016-03) Karmakar, A.; Chakraborty, P.; Sarkar, T.; Das, S.; Saha, A.; Saha, D.; Saha, A.Tomato is a solanaceous crop and one of the most economically important vegetables in the world. India ranks second in total production of tomato in the world. It has been referred to as a “functional food”, a food that goes beyond providing just basic nutrient. To LCD is one of the major constraints to tomato production in India. To study the disease incidence of tomato, a survey was made in the tomato crop growing fields of Darjeeling, Jalpaiguri, Coochbehar and Uttar Dinajpur districts of sub-Himalayan West Bengal during December 2015 to February 2016 and several infected and healthy leaf samples were collected based on the morphological symptoms like-vein cleaning, leaf curling, leaf deformations and stunted growth of plants. Disease incidence ranged from 70% to 86.66% of the collected samples from different districts. All the samples collected from the present study area were tested by PCR with DengA and DengB primer and expected amplicon of ̴ 530bp was found. Two randomly selected PCR positive samples were sequence and analyzed (Acc. Nos KX108859 and KX108860). The SLG-1 isolate (Acc. No. KX108859) showed 95 % nt identity with ToLCKV (Acc. No. KP178730) and the ISL-1 isolate (Acc. No. KX108860) showed 96% nt identity with ToLCNDV (Acc. No. KC513822). The threat of begomoviral spread to the north-eastern part of India has been taken into consideration.