NBU Journal of Plant Sciences, Vol. 11

Permanent URI for this collectionhttps://ir.nbu.ac.in/handle/123456789/4526

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Author: search.filters.author.Sen, Armab

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    Effect of solvents on qualitative and quantitative phytochemical constituent profiles of fresh leaves of TV26
    (University of North Bengal, 2019-03) Labar, Reha; Sen, Armab; Bhattacharya, Malay
    The aim of this study was to qualitatively and quantitatively screen and identify major phytochemical groups from leaves extract of TV26 extracted by nine different solvents having different polarities. Qualitative screening suggested acetone, methanol, ethanol and ethyl acetate to be the most potent solvent for various phytochemical extractions like flavonoid, tannin, steroid, diterpenes, terpenoids, coumarin, cardiac glycoside, saponin, protein and reducing sugar. The highest percent of radical scavenging was recorded in cold water extracts (5mg/ml) i.e. 91.10% and was at par with 93.40% percent scavenging activity of ascorbic acid taken as standard (5mg/ml). Methanol, acetone, ethanol and ethyl acetate gave the best results with the total phenol content value (GAE) recorded as 100.60mg/g, 87.07mg/g, 58.73 mg/g and 51.47mg/g respectively with methanol giving the best result. Acetone extracts (5mg/ml) showed higher ferric reducing power with IC, value 426.45+ 1.12 μg/ml compared to the standard (ascorbic acid) 270.35-0.66 μg/ml. Our findings suggest that the polar solvents were more beneficial and potent against the other non polar counterparts during phytochemical extraction but the polarity of solvents need not be in increasing order since we can assume from our results that acetone being less polar than ethanol, methanol and water showed better results. In addition to different polarities, state of the sample and extraction technique is also crucial for better extraction.
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    RAPD analysis and rDNA gene sequence based phylogeny of Bipolaris sorokiniana, a spot blotch pathogen of sorghum
    (University of North Bengal, 2019-03) Bhattacharjee, Priyanka; Sen, Armab; Chakraborty, Usha; Chakraborty, Biswanath
    Sorghum [Sorghum bicolor (L) Moench] is the one of the most important cereal crops in the world. It is the staple food grain for over 750 million people who live in the semi-arid tropics of Africa, Asia, and Latin America. Global pro-duction of sorghum is currently estimated to be 57.6 million tonnes, with Asian countries contributing 20% of the total production. Within Asia, India is the largest producer of sorghum grain. Recently there have been severe signs of sorghum decline caused by Bipolaris sorokiniana resulting in decreased production of sorghum in villages of Kalimpong and Darjeeling. In the present study, initially, several strains of the fungus were isolated from diseased leaves of Sorghum bicolor and Triticum aestivum which were morphologically identified as Bipolaris sorokiniana. Genomic DNA of B. sorokiniana isolated from infected leaves was purified and PCR amplification of 18s rDNA was done using specific primers. Amplified product (1190 bp) was sequenced and aligned against ex-type strain sequences of B. sorokiniana from NCBI GenBank using BLAST and phylogenetic analysis was done using MEGA4 software. RAPD PCR analysis and DGGE analysis of amplified genomic DNA were done. The evolutionary history was inferred using the UPGMA method. Amplification of ITS region of the rDNA can be considered as a rapid technique for identifying pathogens successfully in all cases.