Browsing by Subject "DPPH"

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Open Access
Free radical scavenging activity of ornamental and edible cultivars of Canna found in Eastern India
(University Of North Bengal, 2011-03) Mishra, T; Goyal, AK; Mondal, P; Sen, A
Canna, the only genus of family Cannaceae, found in eastern India have gained economic importance for their starchy rhizomes and attractive flowers. The rhizome of some edible cultivars are consumed as food and herbal medicines in rural areas. The presence of total phenols, flavonoids and DPPH scavenging activity of both cold and hot extracts and their correlations have been studied for eleven cultivars of Canna spp. Antioxidants, quercetin and gallic acid equivalence of phenolic compounds were determined spectrophotometrically. It has been found that total phenol, flavonoids and DPPH free radical scavenging activity were higher in hot extracts than in cold. Further studies gave an idea about the correlation between phenol and flavonoid contents of both cold and hot extracts, but both the above said parameters were not correlated with DPPH scavenging activity. Finally it can be concluded that higher DPPH scavenging activities in all these Canna cultivars may not be due to the presence of endogenous phenols and flavonoids but these may be due to any other phytochemicals.
Open Access
In vitro antioxidant activity of two edible Timbur fruits of Darjeeling Himalaya
(University of North Bengal, 2010-03) Ghosal, Mitali; Mandal, Palash
Frec radicals are implicated for many chronic, painful and near-fatal diseases including Diabetes mellitus, arthritis, cancer, apoptosis, neurodegenerative disorders etc. In treatment of these discases, antioxidant therapy has gained an utmost importance and current rescarch is now directed towards finding naturaly occurring antioxidants of plant origin. Edible Timburs namely Zanthoxylum acanthopodium DC. and Litsea cubeba (Loureiro) Persoon are traditionally usced as spices in different Nepali recipes. In the present study, the fruits of these plants were extracted with aqucous methanol (1:4) to examinc the in vitro antioxidant property, phenol content and phytochemical constituents. The scavenging activities on DPPH free radicals, superoxide anions and per-oxidized lipid molecules were determined as well as the flavonoid and phenolic constitucnts of the extracts. The extracts exhibited significant scavenging activity towards DPPH free radicals and high anti-lipid peroxidation values duc to the presence of relatively high total phenol contents. Also, these spices contain a vast array of different phytochemicals in their dry form. These results suggest that both Zanthoxylum acanthopodium and Litsea cubeba fruits are endowed with antioxidant phytochemicals and could serve as basal ingredients for nutraceutical formulations.
Open Access
In-vitro free radical scavenging activities of the leaves of Malva verticillara L
(University of North Bengal, 2012-03) Chhetri, P.K.; Mandal, P.
Antioxidants act as major defense against radical-mediated toxicity by protecting against the damages caused by free-radicals. Research on herbal products are increasingly focused on their effects on scavenging of different newly generated free-radical species and associated oxidative stress mediated complications on human health, but there are unexpectedly few studies evaluating the bioactivity of edible leafy vegetables of North Bengal, India. The purpose of the present study was to investigate the in vitro antioxidant potential of methanol extract and aqueous decoction of Malva verticillata L. [MV] leaves, consumed by local people of North Bengal. Extracts of leaves were analyzed for in vitro free radical scavenging capacity, the total phenol and flavonoid content and preliminary phytochemical analysis. The antioxidant property was estimated using reducing power, superoxide radical scavenging activity and DPPH assays. Methanol extract of leaves were found to be effective in DPPH and superoxide radical scavenging activity when compared with aqueous decoction of MV. Overall strong correlation between the mean values of total phenol content and IC50 values of DPPH and superoxide free radical scavenging capacity was observed. Principle Component Analysis (PCA) indicated that phenolic functional groups and reducing potential of methanol and aqueous extracts were mostly contributed for their antioxidant capacity. The present study revealed that methanol extract of the leaves of MV comprise effective source of natural antioxidants, which might be helpful in preventing the progress of various oxidative stress induced diseases.