Browsing by Subject "AM Fungi"
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Item Open Access Arbuscular mycorrhizal fungal association in rhizosphere of Hevea brasiliensis(University of North Bengal, 2009-03) Chakraborty, BN; Sunar, KOccurrence of VAM spores in Hevea brasiliensis growing soils and percent colonization in roots of three varieties (RRII 105, RRIM 600 and GT 1) were studied. Spore population varied from 30 to 45/g soil and the root infection of all the three varieties ranged from 70 to I00 % in both mature and young plants. Percent of root infection was found to be more in the drought resistant varieties RRII 105 followed by RRIM 600 and the less drought resistant variety GT-1. Nine different types of glomalean spores were recovered from the soil samples, including five from the genus Glomus, three from Acaulospora, one from Gigaspora and few undefined species of Sclerocystis. The scanning electron microscopic observations of the most commonly occurring spores of Glomus fasciculatum revealed smooth wall character with number of pits.Item Open Access Association of AM fungi in the rhizosphere of Thuja orientalis (L)(University of North Bengal, 2013-03) De, U.K.; Das Biswas, R.; Chakraborty, B.N.Thuja orientalis (L) is used as an ornamental plant as well as medicinal plant in India from prehistoric times and instances were found in various manuscripts revealing its medicinal property against abdominal pain, hook worms, arthritis etc. AMF associated with rhizosphere of Thuja were extensively studied in relation to their population in soil, root-length colonization, histopathological study as well as their diversification. Predominant existence of various species of Glomus, Gigaspora were determined and their spore surface texture was examined using stereo microscope, besides, species of Acaulospora, Scutellospora etc. were also documented. Histopathological studies of host roots showed various types of hyphal network and arbuscules, AMF were tried to identify up to species level with the help of standard keys. The result indicated that the various spp. of AMF have established their colonization in host roots and the host plant have a significant role in root tissue colonization.Item Open Access Morphological characterization of rice cultivars their root colonization with arbuscular mycorrhizal fungi and screening for field resistance caused by brown spot disease(University of North Bengal, 2015-03) Khati, Sweata; Chakraborty, BishwanathVariability in seed morphology was studied in 15 rice cultivars using qualitative and quantitative characters. Germplasm of these indigenous rice landraces were collected from Bijanbari, Kalimpong, Sikkim, Malda, Siliguri and UBKV (Uttar Banga Krishi Viswavidyalaya). Data were recorded for traits such as Kernel colour, Seed cóat colour, Aroma, Presence of Awn and Length of the seed. A total of 9 landraces had white kernel colour while 4 had brown and 2 had greyed-orange. The seed coat colour variation in different landraces ranged from Golden yellow, Yellow, Red and Black. 6 landraces were having aroma whereas 9 had no aroma and lastly 11 landraces were found to have awn and 4 were awnless. UBKV-4 was longest in length with 1.1 cm and Sano masuri being the smallest of 0.4 cm. Establishment of disease in naturally infected rice cultivars were observed and disease index was calculated. Arbuscular Mycorrhizal Fungi (AMF) were screened from rhizosphere of fifteen rice cultivars grown on experimental field using wet sieving and decanting method. Microscopical observation revealed the presence of different genus of sp. Were found to high in all the fifteen cultivars of rice plants followed by Gigaspora, Scutellospora and Acaulospora. Histopathological study of roots showed the presence of vesicles and arbuscules. AMF infection and total number of spores per 100 grams of soil were recorded. Present study evaluates the study of different AMF population and their histopathology harbouring in the rhizosphere of rice.Item Open Access Physico-chemical analysis of rhizospheric soil, mycorrhizal association and root colonization of Mulberry (Morus alba L) plants grown in Kalimpong hills(University of North Bengal, 2014-03) Acharya, A.; Allay, S.; Ram, R. L.; Chakraborty, B. N.Morus alba L, known as white mulberry, is widely cultivated in the hills and its leaves are the major source of food for the silkwoms (Bombya mori L.) employed in the commereial production of silk. The physico-chemical properties of the soil where this plant is cultivated were analysed. The soils analysed had medium to high nitrogen content, but low to medium phosphorus and potash. Since mycorrhizal fungi can help in nutrient mobilization, AMF associated with the rhizosphere of the mulberry tree cultivated in RSRS, Kalimpong were extensively studied in relation to their population in soil, root colonization as well as their diversity. Predominant species of Glomus and few species of Gigaspora was determined. Histo-pathological studies of host roots showed the presence of vesicles and different kind of hyphal network. AMF spores were tried to be identified up to species level with the belp of standard keys. The result indicates that various species of AMF have established successful colonization with the host plant that will further support and help the plant for better growth and development