Browsing by Author "Dasgupta, S."

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    Immunotechniques: Concept and application in plant pathology
    (University of North Bengal, 2007-03) Saha, A.; Dasgupta, S.; Saha, D.
    Proper management of plant diseases requires accurate disease diagnosis and precise identification of the phytopathogens involved. Traditional and conventional methods of disease diagnosis and pathogen detection have several demerits. It takes a lot of time to diagnose a disease and to identify a pathogen accurately by traditional approaches. Moreover, results are not always conclusive because similar symptoms can be caused by different pathogens or physiological conditions and sometimes it is very difficult to distinguish closely related organisms on the basis of their morphological characters only. Thus the traditional methods normally applied for pathogen identification and disease diagnosis are less sensitive and less reliable. The need for rapid and accurate identification and diagnostic methods to identify the organisms that cause plant diseases are essential for the formulation of effective disease control strategies.
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    Influence of culture media and environmental factors on mycelial growth, sporulation and spore germination behaviour of Curvularia eragrostidis (P. Hennings) Mayer
    (University of North Bengal, 2008-03) Saha, A.; Dasgupta, S.; Mandal, P.; Saha, D.
    Curvularia eragrostidis is a foliar fungal pathogen of young tea plants. It causes leaf spot disease of tea. Mycelial growth, sporulation and spore germination behaviour of the pathogen were studied. Six different media were tested for mycelial growth. Among these, potato carrot agar (PCA) was found best for the mycelial growth and sporulation. Maximum mycelial growth was attained after 15 days of incubation. Mycelial growth was also studied in different temperatures and pH. Optimum temperature of growth was 25 °C and best growth was obtained at pH 6.0. Glucose and peptone were best carbon and nitrogen sources respectively for growth and sporulation of the fungus. The optimum conditions of spore germination were found to be at pH 7.25 and at incubation temperature of 25 °C. Keywords: Sporulation; Curvularia eragrostidis; Mycelial growth, fungus