Browsing by Author "Chakraborty, B. N."

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    Physico-chemical analysis of rhizospheric soil, mycorrhizal association and root colonization of Mulberry (Morus alba L) plants grown in Kalimpong hills
    (University of North Bengal, 2014-03) Acharya, A.; Allay, S.; Ram, R. L.; Chakraborty, B. N.
    Morus alba L, known as white mulberry, is widely cultivated in the hills and its leaves are the major source of food for the silkwoms (Bombya mori L.) employed in the commereial production of silk. The physico-chemical properties of the soil where this plant is cultivated were analysed. The soils analysed had medium to high nitrogen content, but low to medium phosphorus and potash. Since mycorrhizal fungi can help in nutrient mobilization, AMF associated with the rhizosphere of the mulberry tree cultivated in RSRS, Kalimpong were extensively studied in relation to their population in soil, root colonization as well as their diversity. Predominant species of Glomus and few species of Gigaspora was determined. Histo-pathological studies of host roots showed the presence of vesicles and different kind of hyphal network. AMF spores were tried to be identified up to species level with the belp of standard keys. The result indicates that various species of AMF have established successful colonization with the host plant that will further support and help the plant for better growth and development
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    Searching for phosphate solubilizinz tungal isolates from soil
    (University of North Bengal, 2008-03) Chakraborty, B. N.; Chakraborty, U.; Saha, A.; Dey, P. L.; Sunar, K.
    A total of 354 fungal isolates were obtained from soil samples collected from forests, river basins and agricultural fields of North Bengal using serial dilution, direct soil plating, serial root washing and root maceration techniques. Cultural characteristics of the isolated fungi were studied and microscopic observations were made for identification of these isolates. All the isolates were screened for their phosphate solubilizing activities in vitro. A total of 70 fungal isolates showed phosphate solubilizing activities as detected in Pikovskaya's agar medium. Quantitative evaluation of phosphate solubilization in liquid medium supplemented with two phosphate sources (tricalcium phosphate and rock phosphate) was carried out for all the isolates showing phosphate solubilizing activity. Maximum phosphate solubilizing capacity was shown by three isolates of A. niger while A. clavatus showed minimum activity. Genomic DNA was extracted from sixteen isolates showing high activity and PCR amplification of DNA from nine isolates was done.