DSpace Community: NBU Journal of Plant Sciences, An Annual Journal of Department of Botany, University of North BengalNBU Journal of Plant Sciences, An Annual Journal of Department of Botany, University of North Bengalhttp://ir.nbu.ac.in/handle/123456789/43842024-03-28T10:08:17Z2024-03-28T10:08:17ZAntifungal Efficacy of Cu-Based Nano-Chitosan on Rhizopus stolonifer, A Virulent PhytopathogenChouhan, DivyaChoudhuri, ChandraniDutta, PoulamiMandal, PalashMathur, Piyushhttp://ir.nbu.ac.in/handle/123456789/50452023-09-17T08:21:25Z2022-01-01T00:00:00ZTitle: Antifungal Efficacy of Cu-Based Nano-Chitosan on Rhizopus stolonifer, A Virulent Phytopathogen
Authors: Chouhan, Divya; Choudhuri, Chandrani; Dutta, Poulami; Mandal, Palash; Mathur, Piyush
Abstract: Agro-scientists are giving endless efforts for synthesizing a bio-derived molecule that can act as a promising antifungal agent for combating a large number of phytopathogens. Harmful phytopathogens decrease crop yield and its quality. Rhizopus stolonifer is one such virulent phytopathogen that causes huge losses during the post-harvest period of crops. This pathogen mainly causes rot disease in fruits, crops, and vegetables. The second most abundantly available biological macromolecule, Chitosan and its metal-based nanoparticles stands as a potential antifungal agent for combating Rhizopus stolonifer. This study includes the synthesis of Cu chitosan nanoparticles (Cu-CNPs) and chitosan nanoparticles (CNPs) through the ionic gelation method and its characterization based on UV Vis spectrophotometer, FE-SEM, EDXS, and DLS. Cu-CNPs and CNPs were screened from 100-2000 µg/mL concentration against R. stolonifer for the assessment of its antifungal activity. Spore viability assay and lipid peroxidation of the pathogen using Cu-CNPs and CNPs were also determined. Generation of oxidative stress in the mycelium of the pathogen on the application of Cu-CNPs and CNPs was traced by fluorescence microscopy. Changes in the ultra-structure of the sporangium of R. stolonifer after treatment with Cu-CNPs and CNPs were visualized under SEM. Results showed that Cu-CNPs inhibit the growth of R. stolonifer at 2000 µg/mL and elevate malonaldehyde (MDA) content in the pathogen as a result of lipid peroxidation and produces defined damages on the sporangium membrane as observed under electron microscope. Fluorescence microscopy revealed the emission of high intensity of fluorescence due to the generation of oxidative stress in Cu-CNPs treated fungal mycelium.2022-01-01T00:00:00ZPolyethylene Gycol (PEG) Induced Water Stress in Four Different Genotypes of Pea Seedlings and Evaluation of The Induced Defense MechanismBantawa, PranayRai, Rituhttp://ir.nbu.ac.in/handle/123456789/50442023-09-17T08:17:15Z2022-01-01T00:00:00ZTitle: Polyethylene Gycol (PEG) Induced Water Stress in Four Different Genotypes of Pea Seedlings and Evaluation of The Induced Defense Mechanism
Authors: Bantawa, Pranay; Rai, Ritu
Abstract: Present work was undertaken on artificially induced water stress on pea seedlings under in vitro conditions in order to select the drought tolerant line. Artificial water stress was induced with PEG-6000 on 15-day old seedlings of four varieties for 4, 8 and 12 days. The activities of antioxidative enzymes like peroxidase, catalase, ascorbate peroxidase, glutathione reductase and superoxide dismutase were assayed in the stressed and control plants. POX activity was increased in the initial stages of stress, but its activity was decreased significantly on the 12th day in all the varieties. APOX also showed a similar trend but the maximum activity was noted in Var 3 on the 8th day. CAT activity decreased in var 1 and var 2 when compared with the control, which, however, increased significantly in var 3 and var 4. A slight increase in the GR activity was observed in var 1 and var 2 at the initial stages of the drought stress but its activity decreased significantly on the 12th day in both these varieties when compared with control plants. However, its activity also increased steadily in var 3 and var 4. Maximum SOD activities were noted on the 4th day of drought stress in all four varieties but its activities decreased steadily on the subsequent 8th and 12th days when compared with control. When antioxidative activities were compared among the four varieties, var 3 and var 4 showed maximum increase in antioxidant activity during the period of drought stress. Among the four varieties, var 3 and var 4 showed greater accumulation of H2O2 during the stress days and were maximum at 12th day. Lipid peroxidation also increased in the same varieties. Maximum proline content was noted in both the root and leaf of var 3, followed by var 4. It was further noted that the chlorophyll content decreased significantly in all four varieties in subsequent longer drought stresses. The accumulation of proline content was steadily higher with an increase in the stress length in all the four varieties. During the drought stress, all the varieties showed an increase in ascorbate content but, it was maximum in var 4 followed by var 3 and the least ascorbate was noted in var 1. The present findings indicate that water stress induces oxidative stress in all the four varieties. However, antioxidative mechanisms were found to be more pronounced in var 4 which, therefore, may be considered as the most tolerant to drought stress.2022-01-01T00:00:00ZPhytoResp : A Database for Medicinal Plants of Darjeeling, Against Respiratory AilmentsDas, AnushkaBanik, AnanyaDatta, SutapaSen, Arnabhttp://ir.nbu.ac.in/handle/123456789/50432023-09-17T08:15:07Z2022-01-01T00:00:00ZTitle: PhytoResp : A Database for Medicinal Plants of Darjeeling, Against Respiratory Ailments
Authors: Das, Anushka; Banik, Ananya; Datta, Sutapa; Sen, Arnab
Abstract: PhytoResp is a digital, manually curated database developed at the Department of Botany, University of North Bengal (NBU). The database has been created with the help of 200 published research articles and the eleven volumes of ‘Chiranjib Banousadhi’ series, written by Shibakali Bhattacharya. Here, we provide the names of 329 medicinal plants that are available in the Darjeeling district region of North Bengal and are traditionally used in the treatment of respiratory diseases. The list of reported plants is included, along with their parts used. They are known to cure 38 respiratory diseases. Among these, 113 plants have already been reported as a cure for COVID-19 (in silico or in vivo). Also, we have taken a step to evaluate the efficiency of the phytocompounds through in-silico methods.2022-01-01T00:00:00ZConcept of The Genus Acampe (Lindl.) in IndiaChakraborty, OindrilaAgrawala, Dinesh KumarChakraborty, Arka Pratimhttp://ir.nbu.ac.in/handle/123456789/50422023-09-20T07:23:12Z2022-01-01T00:00:00ZTitle: Concept of The Genus Acampe (Lindl.) in India
Authors: Chakraborty, Oindrila; Agrawala, Dinesh Kumar; Chakraborty, Arka Pratim
Abstract: Genus Acampe Lindl. (Orchidaceae) has been taxonomically studied in Indian context. Out of 10 species of Acampe found globally, four species and one variety were recognized in India. Detailed taxonomic account including description, illustrative photo-plate along with information on phenology, habitat and distribution have been provided. The threat status of each taxon has been assessed in Indian perspective by following the IUCN guidelines.2022-01-01T00:00:00Z