Browsing by Subject "Lipid Peroxidation"

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Open Access
Antifungal Efficacy of Cu-Based Nano-Chitosan on Rhizopus stolonifer, A Virulent Phytopathogen
(University of North Bengal, 2022) Chouhan, Divya; Choudhuri, Chandrani; Dutta, Poulami; Mandal, Palash; Mathur, Piyush
Agro-scientists are giving endless efforts for synthesizing a bio-derived molecule that can act as a promising antifungal agent for combating a large number of phytopathogens. Harmful phytopathogens decrease crop yield and its quality. Rhizopus stolonifer is one such virulent phytopathogen that causes huge losses during the post-harvest period of crops. This pathogen mainly causes rot disease in fruits, crops, and vegetables. The second most abundantly available biological macromolecule, Chitosan and its metal-based nanoparticles stands as a potential antifungal agent for combating Rhizopus stolonifer. This study includes the synthesis of Cu chitosan nanoparticles (Cu-CNPs) and chitosan nanoparticles (CNPs) through the ionic gelation method and its characterization based on UV Vis spectrophotometer, FE-SEM, EDXS, and DLS. Cu-CNPs and CNPs were screened from 100-2000 µg/mL concentration against R. stolonifer for the assessment of its antifungal activity. Spore viability assay and lipid peroxidation of the pathogen using Cu-CNPs and CNPs were also determined. Generation of oxidative stress in the mycelium of the pathogen on the application of Cu-CNPs and CNPs was traced by fluorescence microscopy. Changes in the ultra-structure of the sporangium of R. stolonifer after treatment with Cu-CNPs and CNPs were visualized under SEM. Results showed that Cu-CNPs inhibit the growth of R. stolonifer at 2000 µg/mL and elevate malonaldehyde (MDA) content in the pathogen as a result of lipid peroxidation and produces defined damages on the sporangium membrane as observed under electron microscope. Fluorescence microscopy revealed the emission of high intensity of fluorescence due to the generation of oxidative stress in Cu-CNPs treated fungal mycelium.
Open Access
Comparative study of basal thermo tolerant attributes in tolerant and susceptible wheat cultivars
(University of North Bengal, 2019-03) Sarkar, Jayanwita; Chakraborty, Usha; Chakraborty, Bishwanath
Two wheat cultivars, viz. C306 and HT41, have been evaluated for their responses to heat stress in terms of some biochemical and physiological attributes. During the exposure to high temperature (40°C for 6 and 12 h), a significant reduction in RWC and FW was recorded in HT41 which was also associated with heat susceptibility. The detrimental effects of high temperature were also apparent in terms of lipid peroxidation, chlorophyll content, H2O2 generation and electrolyte leakage and these were more pronounced in HT 41. Accumulation of osmolytes like proline, total sugar increased due to heat injury and also varied between the two cultivars. In this present study evaluation and analysis of these biochemical and physiological characters under heat stress could unravel the mechanism of basal thermotolerance and thus might be useful as genetic stock to develop wheat tolerant varieties.